mouse anti human anti ccr7 percp  (R&D Systems)

Journal: Cell Reports Medicine

Article Title: Th2-like T Follicular Helper Cells Promote Functional Antibody Production during Plasmodium falciparum Infection

doi: 10.1016/j.xcrm.2020.100157

Figure Lengend Snippet: Memory Phenotypes of Tfh Subsets during Infection (A) cTfh (PD1 + CXCR5 + CD4) cells were differentiated into memory phenotypes based on CD45RA and CCR7 expression into central memory (CM CD45RA − CCR7 + ), effector memory (EM CD45RA − CCR7 − ), terminal differentiated effector memory (TEMRA CD45RA + CCR7 − ), and naive (CD45RA + CCR7 + ) phenotypes. (B) cTfh subsets (Th1, Th2, Th17, based on CXCR3 and CCR6 expression) had different distributions of memory phenotypes. (C) Mean memory phenotypes across subsets at day 0, before inoculation. (D) Proportion of CM and EM phenotypes within cTfh subsets after infection. Flow cytometry-based assays were performed once on cryopreserved PBMC samples tested in singles. Data are from 6 individual study participants from days 0, 8, and EOS (cryopreserved PBMC day 14/15 samples were not available). (A) and (B) are representative data from 1 individual. (C) is the median of each memory phenotype in each subset. (D) Boxplots are Tukey style, with median and IQR. Whiskers are to data point no further than 1.5 × IQR, dots are outlying points. Paired data are joined by lines. Wilcoxon signed-rank test compared to day 0 are indicated.

Article Snippet: Mouse anti-human CCR7 PerCP-Cy5.5 (Clone 150503) , BD Biosciences , Cat#561144; RRID: AB_10562553.

Techniques: Infection, Expressing, Flow Cytometry

Journal: Cell Reports Medicine

Article Title: Th2-like T Follicular Helper Cells Promote Functional Antibody Production during Plasmodium falciparum Infection

doi: 10.1016/j.xcrm.2020.100157

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CCR7 PerCP-Cy5.5 (Clone 150503) , BD Biosciences , Cat#561144; RRID: AB_10562553.

Techniques: In Vitro, Recombinant, Binding Assay, Purification, Software

Journal: Journal of Immunology Research

Article Title: Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes

doi: 10.1155/2016/6031486

Figure Lengend Snippet: Characterization of M1- and M2-related phenotypes. Median fluorescence intensity corresponding to (a) M1-related markers TNF- α , IDO enzyme, CCR7, and CD86 and (b) M2-related markers IL-10, arginase I, CD36, and CD163, found in cells harvested from all different polarizing treatments and controls and analyzed by flow cytometry. For THP-1, mock: no stimulated cells; PMA: PMA-only control; M1: PMA + LPS + INF- γ ; M2: PMA + IL-4 + IL-13; and M2 w/o PMA: IL-4 + IL-13. For U937, mock: no stimulated cells; PMA: PMA-only control; M1: PMA + LPS + INF- γ ; M2-A: PMA + IL-4 + IL-13; M2-B: PMA + M-CSF; and M2-C: IL-4 + IL-13. For primary monocytes, mock: no stimulated cells; M1: GM-CSF + LPS + INF- γ ; and M2: M-CSF + IL-4 + IL-13. Asterisks and bars denote statistical significance between conditions. Significant differences ( p ≤ 0.05) found were as follows: in THP-1 cells CD86, M1 versus M2; IL-10, mock versus all the rest of the conditions; arginase I, M1 versus PMA and versus M2 without PMA; CD163, PMA versus all the rest of the conditions and M2 versus M2 without PMA. In U937 cells IDO, M2-A versus mock and versus M2-C; CD86, (PMA, M1, M2-A, and M2-B) versus mock and M2-C; CD163, M1 versus mock and versus M2-C. In primary monocytes IDO and CD86 were significantly different in M1 versus mock and versus M2.

Article Snippet: After incubation in the blocking solution 100 μ L of a 1 : 100 dilution in blocking solution of antibodies was added (for panel 1 staining: mouse anti-human anti-CD86-PE and anti-CD163-peridinin­chlorophyll protein complex (PerCP); for panel 2 staining: rat anti-human anti-CD36-fluorescein and mouse anti-human anti-CCR7-PerCP; all antibodies from R&D Systems) and incubated at 4°C for 30 minutes.

Techniques: Fluorescence, Flow Cytometry